Antigenicity of the Human Zona Pellucida

Abstract

Antiserum produced against saline homogenates of human ovary yielded a minimum of 9 precipitin bands when reacted against human ovary in double diffusion analyses. This antiserum also cross reacted in varying degrees when tested against 21 other human tissues and fluids. The anti-human ovary serum could be rendered specific to human ovary by absorption with human liver and plasma. Both the unabsorbed and absorbed antisera reacted with isolated human zonae pellucidae as demonstrated by the formation of a precipitation layer on the zona and by the indirect fluorescent antibody technique.