Echinocandin inhibition of 1,3‐β‐D‐glucan synthase from Candida albicans
- 23 July 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 173 (1), 134-138
- https://doi.org/10.1016/0014-5793(84)81032-7
Abstract
The cyclic peptide antibiotic echinocandin was found to inhibit 1,3-beta-D-glucan synthase activity present in a mixed membrane fraction from Candida albicans. Addition of antibiotic reduced the Vmax of the enzyme, but the Km was unaffected. GTP stimulated enzyme activity approximately 4-fold, but did not affect the percentage inhibition of the enzyme by echinocandin. Treatment of the reaction products with alpha-amylase and beta-glucanase confirmed that the polymer synthesised was 1,3-beta-D-glucan, not glycogen.Keywords
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