Action of Adenine and Related Compounds on Replication of Encephalomyocarditis Virus in Tissue Culture.

Abstract

The addition of 100-500 [mu]gm/ml of adenine under a variety of experimental conditions to Krebs 2 and Ehrlich ascites tumor cell suspension in a simplified medium was found to delay the growth of encephalomyocarditis virus. The approximately 2-log lower yield in both supernatant and cell-associated virus observable at 24 hours was less apparent after this time. The replication of EMC virus in L cells as well as influenza, PR8, in CAM roller tubes, and de-embryonated eggs was unaffected by adenine under the experimental conditions so far tested. Other related compounds were essentially without effect at non toxic levels.