Action of Adenine and Related Compounds on Replication of Encephalomyocarditis Virus in Tissue Culture.
- 1 January 1962
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 109 (1), 82-85
- https://doi.org/10.3181/00379727-109-27110
Abstract
The addition of 100-500 [mu]gm/ml of adenine under a variety of experimental conditions to Krebs 2 and Ehrlich ascites tumor cell suspension in a simplified medium was found to delay the growth of encephalomyocarditis virus. The approximately 2-log lower yield in both supernatant and cell-associated virus observable at 24 hours was less apparent after this time. The replication of EMC virus in L cells as well as influenza, PR8, in CAM roller tubes, and de-embryonated eggs was unaffected by adenine under the experimental conditions so far tested. Other related compounds were essentially without effect at non toxic levels.Keywords
This publication has 5 references indexed in Scilit:
- METHODS FOR MEASURING VIABILITY OF ASCITES CELLS DYE EXCLUSION AND RESPIRATION AS AFFECTED BY DEPLETION, POISONS, AND VIRUSES1959
- The Ability of Purine and Thymine Derivatives and of Glycine to Support the Growth of Mammalian Cells in CultureJournal of Biological Chemistry, 1959
- Chemical Inhibitors of Theiler's Virus.Experimental Biology and Medicine, 1956
- A Serum-Free Nutrient Solution Sustaining Rapid and Continuous Proliferation of Strain L (Earle) Mouse Cells2JNCI Journal of the National Cancer Institute, 1956
- The effect of purines and related substances upon cells in chick tissue culturesExperimental Cell Research, 1952