Effects of Phytohemagglutinin on Rat and Normal and Leukemic Human Blood Cells.
- 1 May 1963
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 113 (1), 191-194
- https://doi.org/10.3181/00379727-113-28317
Abstract
Summary Suspensions of normal human blood cells were incubated at 37°C with phytohemagglutinin (PHA) in slide chambers. In 2 to 4 days, the reagent stimulated the formation of large blast-like cells (PHA-cells) which could be readily differentiated from macrophages. PHA-cells developed in purified suspensions of lymphocytes and many intermediate cells were seen between PHA-cells and lymphocytes. PHA-cells did not develop in blood cell suspensions of some patients with chronic lymphocytic leukemia nor in suspensions of rat blood cells. The findings suggest that PHA-cells were derived from normal human lymphocytes and should be differentiated from macrophages.Keywords
This publication has 8 references indexed in Scilit:
- Studies of Cell Source of Macrophages from Human Blood in Slide Chambers.Experimental Biology and Medicine, 1962
- The Kinetics of Cell Proliferation in Cultures of Human Peripheral BloodBlood, 1962
- PRIMARY CULTURES OF MACROPHAGES FROM NORMAL HUMAN PERIPHERAL BLOOD1962
- Differences Between Lymphocytes of Leukemic and Non-Leukemic Patients with Respect to Morphologic Features, Motility, and Sensitivity to Guinea Pig SerumBlood, 1961
- FACTORS AFFECTING THE ADHESIVENESS OF HUMAN LEUCOCYTES AND PLATELETS IN VITROThe Journal of Experimental Medicine, 1961
- Differentiation of human leukemic leukocytes in tissue cultureExperimental Cell Research, 1960
- PHYTOHEMAGGLUTININ - AN INITIATOR OF MITOSIS IN CULTURES OF NORMAL HUMAN LEUKOCYTES1960
- SLIDE-CHAMBER METHOD TO MEASURE SENSITIVITY OF CELLS TO TOXIC AGENTS - APPLICATION OF THE METHOD TO NORMAL AND LEUKEMIC HUMAN LYMPHOCYTES1958