Two‐dimensional 1H‐NMR study of the 1–34 fragment of human parathyroid hormone

Abstract

The complete assignment of resonances in the proton nmr spectrum of the 1–34 amino acid fragment of human parathyroid hormone [hPTH(1–34)], determined using a combination of one- and two-dimensional nmr techniques at 500 MHz, is described. In particular, homonuclear Hartmann–Hahn experiments, recorded in H₂O and D₂O, are used to resolve ambiguities in the connectivities between the highly overlapped resonances in the aliphatic region of the spectrum. One-dimensional multiple quantum filtering experiments are used to identify serine and phenylalanine spin systems. Analyses of the through-bond and through-space connectivities in the αH-NH fingerprint regions of the correlated spectroscopy (COSY) and nuclear Overhauser effect spectroscopy (NOESY) spectra lead to the assignment of resonances to specific amino acid residues in the polypeptide. Examination of the observed NOE cross peaks indicates that hPTH(1–34) has no detectable secondary structural elements in aqueous solution.