Multiple Sample PCR Amplification and Electrophoretic Analysis on a Microchip
- 6 November 1998
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 70 (24), 5172-5176
- https://doi.org/10.1021/ac980447e
Abstract
Polymerase chain reactions (PCRs) were carried out on as many as four DNA samples at a time on a microchip device. The PCR products were then analyzed, either individually or together on the same device, by microchip gel electrophoresis. A standard PCR protocol was used to amplify 199- and 500-base pair (bp) regions of bacteriophage λ DNA and 346- and 410- bp regions of E. coli genomic and plasmid DNAs, respectively. Thermal lysis of the bacteria was integrated into the PCR cycle. A product sizing medium, poly(dimethylacrylamide), and an intercalating dye for fluorescence detection were used in the electrophoretic analysis of the products. PCR product sizes were determined by coelectrophoresis with marker DNA.Keywords
This publication has 18 references indexed in Scilit:
- Degenerate Oligonucleotide Primed–Polymerase Chain Reaction and Capillary Electrophoretic Analysis of Human DNA on Microchip-Based DevicesAnalytical Biochemistry, 1998
- Integrated Cell Isolation and Polymerase Chain Reaction Analysis Using Silicon Microfilter ChambersAnalytical Biochemistry, 1998
- Microchip Device for Cell Lysis, Multiplex PCR Amplification, and Electrophoretic SizingAnalytical Chemistry, 1998
- Integrated Capillary Electrophoresis Devices with an Efficient Postcolumn Reactor in Planar Quartz and Glass ChipsAnalytical Chemistry, 1996
- Functional Integration of PCR Amplification and Capillary Electrophoresis in a Microfabricated DNA Analysis DeviceAnalytical Chemistry, 1996
- Microchip-based devices for molecular diagnosis of genetic diseasesMolecular Diagnosis, 1996
- Integrated Microdevice for DNA Restriction Fragment AnalysisAnalytical Chemistry, 1996
- Precolumn Reactions with Electrophoretic Analysis Integrated on a MicrochipAnalytical Chemistry, 1994
- Microchip Capillary Electrophoresis with an Integrated Postcolumn ReactorAnalytical Chemistry, 1994
- Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplificationNucleic Acids Research, 1988