Herpes simplex virus helicase-primase: the UL8 protein is not required for DNA-dependent ATPase and DNA helicase activities
- 1 January 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (12), 3573-3578
- https://doi.org/10.1093/nar/18.12.3573
Abstract
The herpes simplex virus type 1 helicase-primase complex consists of the products of the UL5, UL8 and UL52 genes. We have expressed these proteins in insect cells using baculovirus vectors and studied the requirements for enzymatic activities associated with the DNA unwinding function of the complex. In agreement with a recent report (Dodson, M.S., Crute, J.J., Bruckner, R.C. and Lehman, I.R. 1989, J. Biol. Chem. 264, 20835-20838) we find that DNA-dependent ATPase and DNA helicase activities are assembled in vivo in insect cells triply infected with viruses expressing the UL5, UL8 and UL52 proteins. Moreover, these activities, were also detected in cells in which only the UL5 and UL52 products were expressed indicating that the presence of the UL8 protein is essential for neither the ATPase nor helicase activity of the complex.This publication has 48 references indexed in Scilit:
- Binding of the herpes simplex virus type 1 UL9 gene product to an origin of viral DNA replicationNucleic Acids Research, 1989
- Baculoviruses as Gene Expression VectorsAnnual Review of Microbiology, 1988
- Herpes simplex virus DNA replication: the UL9 gene encodes an origin-binding protein.Proceedings of the National Academy of Sciences, 1988
- A DNA helicase induced by herpes simplex virus type 1Nucleic Acids Research, 1988
- The Complete DNA Sequence of the Long Unique Region in the Genome of Herpes Simplex Virus Type 1Journal of General Virology, 1988
- Genetic and phenotypic characterization of mutants in four essential genes that map to the left half of HSV-1 UL DNAVirology, 1987
- Baculovirus Expression Vectors: the Requirements for High Level Expression of Proteins, Including GlycoproteinsJournal of General Virology, 1987
- Identification of the N and NSs proteins coded by the ambisense S RNA of punta toro phlebovirus using monospecific antisera raised to baculovirus expressed N and NSs proteinsVirology, 1987
- DNA sequence of the region in the genome of herpes simplex virus type 1 containing the genes for DNA polymerase and the major DNA binding proteinNucleic Acids Research, 1985
- Fragments from both termini of the herpes simplex virus type 1 genome contain signals required for the encapsidation of viral DNANucleic Acids Research, 1983