Production of alkaloids and differentiation in a submerged culture ofClaviceps purpurea (Fr.) Tul
- 1 March 1975
- journal article
- Published by Springer Nature in Folia Microbiologica
- Vol. 20 (2), 112-117
- https://doi.org/10.1007/bf02876766
Abstract
The submerged culture of theClaviceps purpurea strain studied was polymorphous. In. the process of alkaloid synthesis, cytodifferentiation preceded biochemical differentiation. The onset of the alkaloid phase was characterized by. predominance of chlamydospores in the culture, the presence of vegetative cells with reduced or arrested proliferation, maximum acetylCoA carboxylase activity, the maximum amount of total fatty acids, an over-average cell pool tryptophan level and minimum tryptophan synthetase activity. Intracellular ricinoleic acid was an indicator of differentiation of the culture towards alkaloid formation and also of alkaloid synthesis. The cytodifferentiation period in the initial phases of fermentation, when the cell has several alternative possibilities of development, is regarded as the most sensitive sector of the regulatory mechanisms of alkaloid formation.Keywords
This publication has 15 references indexed in Scilit:
- The physiology of conidial formation in submerged cultures of Claviceps purpurea (Fr.) Tul producing alkaloidsCanadian Journal of Microbiology, 1974
- Modification of the sporulation cycle in Penicillium digitatum (Sacc.)Canadian Journal of Microbiology, 1973
- Physiological status of submergedClaviceps during enzymic assembly of ergot alkaloidsFolia Microbiologica, 1972
- Correlation of certain alterations in metabolic activity with alkaloid production by submerged Claviceps.1971
- Cell-pool tryptophan phases in ergot alkaloid fermentationFolia Microbiologica, 1971
- Fatty acid composition of sphacelial and sclerotial growth forms of Claviceps purpurea in relation to the production of ergoline alkaloids in cultureTransactions of the British Mycological Society, 1969
- The Biosynthesis of Ricinoleic Acid by Claviceps purpureaBiochemical Journal, 1966
- Production of ergotamine by a strain ofClaviceps purpurea (Fr.) Tul.Cellular and Molecular Life Sciences, 1966
- [30] Tryptophan synthetase from neurosporaMethods in Enzymology, 1955
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951