groEL and dnaK genes of Escherichia coli are induced by UV irradiation and nalidixic acid in an htpR+-dependent fashion.
- 1 March 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (5), 1499-1503
- https://doi.org/10.1073/pnas.81.5.1499
Abstract
Two proteins with MW of 61,000 and 73,000 were induced by UV light in E. coli mutants in which the SOS responses are constitutively expressed. The induction of these proteins by UV light and nalidixic acid is independent on the recA+ lexA+ regulatory system. Analysis of these proteins by 2-dimensional gel electrophoresis and comparison with the heat-shock proteins of E. coli revealed that the MW 61,000 protein comigrated with the groEL gene product, that the MW 73,000 protein comigrated with the dnaK gene product and that other heat-shock proteins was also induced. The induction of groEL and dnaK by UV light and nalidixic acid is controlled by the htpR locus. The regulatory response of E. coli to agents such as UV light and nalidixic acid is apparently more complex than previously thought.Keywords
This publication has 36 references indexed in Scilit:
- Superhelical Escherichia coli DNA: Relaxation by coumermycinJournal of Molecular Biology, 1978
- A mutant of Escherichia coli showing constitutive expression of the lysogenic induction and error-prone DNA repair pathways.Proceedings of the National Academy of Sciences, 1977
- An adaptive response of E. coli to low levels of alkylating agent: Comparison with previously characterised DNA repair pathwaysMolecular Genetics and Genomics, 1977
- DNA synthesis inhibition and the induction of protein X in Escherichia coliJournal of Molecular Biology, 1976
- Two-dimensional gel electrophoresis of membrane proteinsBiochemistry, 1976
- A temperature sensitive nonsense mutation affecting the synthesis of a major protein of Escherichia coli K12Molecular Genetics and Genomics, 1975
- High resolution two-dimensional electrophoresis of proteins.1975
- Protein synthesis in salivary glands of Drosophila melanogaster: Relation to chromosome puffsJournal of Molecular Biology, 1974
- Prophage induction and cell division in E. coliMolecular Genetics and Genomics, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970