Polyethylene Glycols as Embedding Media in Histochemical Work
- 1 November 1952
- journal article
- research article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 5 (4), 345-349
- https://doi.org/10.1136/jcp.5.4.345
Abstract
Appropriately fixed tissues 2-3 mm. thick are blotted and immersed in 2 changes of "carbo-wax 1000" at 40[degree]C for 1 hr. each and then embedded in freshly melted "carbowax 1000". The blocks are enclosed in a petri dish and cooled for 15 mins. in an ice box. Mounted sections are dried overnight at 37[degree]C. Glycogen, fat, nucleoproteins, acid and alkaline phosphatase, oxidase and peroxidase are demonstrable histochemically in the sections. Blocks are preserved after wrapping in cellophane paper in an air-tight glass jar, preferably in an ice box.Keywords
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