Partial Purification of Bovine Intestinal Alkaline Phosphatase.

Abstract

The microsome fraction of homogenates of intestinal mucosa was isolated by differential centrifugation. In confirmation of previous reports, this fraction contains almost all of the alkaline phosphatase, with a specific activity between 3 and 5 times that of the original homogenate. A soluble prepn. may be obtained by treating a microsome suspension with n-butanol, according to the method of R. K. Morton. The behavior of the soluble prepn. on acetone precipitation is reported, as well as filter paper electrophoresis results with the same prepn. Both types of examination indicate that less than 10% of the soluble prepn. can be phosphatase, but it appears to be particularly suitable for further fractionation.