Detection of Respiratory Syncytial Virus in Nasal Secretions from Infants by Enzyme-Linked Immunosorbent Assay

Abstract
An enzyme-linked immunosorbent assay (ELISA) was developed for detection of respiratory syncytial virus (RSV) in nasal secretions from infants with respiratory disease. RSV was detected in 23 of 29 secretions positive for RSV by tissue culture and in one of 36 samples negative for RSV by tissue culture. The ELISA was a simple, rapid, and surprisingly sensitive test for identification of RSV infection in infants.