Uncoupling of osmoregulation of the Escherichia coli K-12 ompF gene from ompB-dependent transcription
- 1 July 1985
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 163 (1), 82-87
- https://doi.org/10.1128/jb.163.1.82-87.1985
Abstract
The expression of the genes for the Escherichia coli K-12 outer membrane proteins, ompF and ompC, is subject to osmoregulation and responds to changes in the osmolarity of the growth medium. The transcription of these genes is dependent on the products of the regulatory locus ompB (comprising the genes ompR and envZ). The native promoter of ompF was replaced with an inducible lpp promoter to eliminate this transcriptional dependence of ompF expression on ompB. As a result, it was possible for the OmpF protein to be produced in an ompB mutant strain that does not normally express ompF. Surprisingly, the expression of ompF under the lpp promoter was still osmoregulated not only in the ompB+ strain but also in two ompB strains tested. These results indicate the involvement of a factor(s) besides the ompR and envZ gene products in the osmoregulation of OmpF production. This factor may interact with a sequence downstream of the ompF promoter. In addition, we show that the expression of ompF under the lpp promoter has no direct effect on ompC expression. ImagesThis publication has 34 references indexed in Scilit:
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