Inhibition of malignant transformation in vitro by inhibitors of poly(ADP-ribose) synthesis.
- 1 January 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (1), 243-247
- https://doi.org/10.1073/pnas.81.1.243
Abstract
Malignant transformation in vitro of hamster embryo cells and mouse [embryo fibroblast] C3H 10T1/2 cells by X-rays, UV light and chemical carcinogens was inhibited by benzamide and by 3-aminobenzamide at concentrations that are specific for inhibition of poly(ADP-ribose) formation. These compounds slow the ligation stage of repair of X-ray and alkylation damage but not of UV light damage. At high concentrations they also inhibited de novo synthesis of DNA purines and DNA methylation by S-adenosylmethionine. The suppression of transformation by the benzamides is in striking contrast to their reported effectiveness in enhancing sister chromatid exchange, mutagenesis and killing in cells exposed to alkylating agents. Mechanisms regulating malignant transformation are probably different from those regulating DNA repair, sister chromatid exchange and mutagenesis, and may be associated with changes in gene regulation and expression caused by alterations in poly(ADP-ribosyl)ation.Keywords
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