The use of on-line high-performance liquid chromatography-mass spectrometry for the identification of ranitidine and its metabolites in urine
- 1 January 1981
- journal article
- research article
- Published by Taylor & Francis in Xenobiotica
- Vol. 11 (12), 831-840
- https://doi.org/10.3109/00498258109045320
Abstract
1. Reverse and normal phase h.p.l.c. systems have been developed for separation of ranitidine and three metabolites, desmethylranitidine, ranitidine-S-oxide and ranitidine-N-oxide. 2. These h.p.l.c. systems have been evaluated for characterization of ranitidine and metabolites using an h.p.l.c. coupled to a mass spectrometer with a moving belt interface. 3. Ranitidine and its metabolites were thermally degraded under the conditions required to evaporate the reverse phase eluent that contained 40% aq. 0.05 M ammonium acetate. 4. The normal phase eluent was evaporated in the interface at a lower temp, and satisfactory mass spectra were obtained from 1 μg of ranitidine and each metabolite injected on to the h.p.l.c. column. 5. Normal phase h.p.l.c.-mass spectrometry has been used to identify ranitidine and three of its metabolites in rabbit and human urine obtained after oral administration of ranitidine.This publication has 6 references indexed in Scilit:
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