Generation of Naive-Like Porcine-Induced Pluripotent Stem Cells Capable of Contributing to Embryonic and Fetal Development
- 1 February 2013
- journal article
- research article
- Published by Mary Ann Liebert Inc in Stem Cells and Development
- Vol. 22 (3), 473-482
- https://doi.org/10.1089/scd.2012.0173
Abstract
In pluripotent stem cells (PSCs), there are 2 types: naive and primed. Only the naive type has the capacity for producing chimeric offspring. Mouse PSCs are naive, but human PSCs are in the primed state. Previously reported porcine PSCs appear in the primed state. In this study, putative naive porcine-induced pluripotent stem cells (iPSCs) were generated. Porcine embryonic fibroblasts were transduced with retroviral vectors expressing Yamanaka's 4 genes. Emergent colonies were propagated in the presence of porcine leukemia inhibitory factor (pLIF) and forskolin. The cells expressed pluripotency markers and formed embryoid bodies, which gave rise to cell types from all 3 embryonic germ layers. The naive state of the cells was demonstrated by pLIF dependency, 2 active X chromosomes (when female), absent MHC class I expression, and characteristic gene expression profiles. The porcine iPSCs contributed to the in vitro embryonic development (11/24, 45.8%) as assessed by fluorescent markers. They also contributed to the in utero fetal development (11/71, 15.5% at day 23; 1/13, 7.7% at day 65). This is the first demonstration of macroscopic fluorescent chimeras derived from naive-like porcine PSCs, although adult chimeras remain to be produced.Keywords
This publication has 59 references indexed in Scilit:
- A Murine ESC-like State Facilitates Transgenesis and Homologous Recombination in Human Pluripotent Stem CellsCell Stem Cell, 2010
- Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCsProceedings of the National Academy of Sciences, 2010
- Conserved and Divergent Roles of FGF Signaling in Mouse Epiblast Stem Cells and Human Embryonic Stem CellsCell Stem Cell, 2010
- Epigenetic reversion of post-implantation epiblast to pluripotent embryonic stem cellsNature, 2009
- Porcine pluripotency cell signaling develops from the inner cell mass to the epiblast during early developmentDevelopmental Dynamics, 2009
- Derivation of induced pluripotent stem cells from pig somatic cellsProceedings of the National Academy of Sciences, 2009
- Generation of Pig Induced Pluripotent Stem Cells with a Drug-Inducible SystemJournal of Molecular Cell Biology, 2009
- Generation of Induced Pluripotent Stem Cell Lines from Tibetan Miniature PigJournal of Biological Chemistry, 2009
- Metastable Pluripotent States in NOD-Mouse-Derived ESCsCell Stem Cell, 2009
- Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined FactorsCell, 2006