Covalent labeling of protein components of the sodium channel with a photoactivable derivative of scorpion toxin.
- 1 January 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (1), 639-643
- https://doi.org/10.1073/pnas.77.1.639
Abstract
Azidonitrobenzoyl mono[125I]iodo scorpion [Leivrus-quinquestriatus] toxin can be covalently attached to its receptor site in electrically excitable neuroblastoma cells [mouse N18] or synaptosomes by photolysis. A polypeptide of MW .apprxeq. 250,000 is specifically labeled in neuroblastoma cells. Labeling is blocked by unlabeled scorpion toxin and by depolarization. This polypeptide is not labeled in a variant neuroblastoma clone lacking voltage-sensitive Na channels. Polypeptides of MW .apprxeq. 250,000 and MW .apprxeq. 32,000 are specifically labeled in synaptosomes. This labeling is also blocked by unlabeled toxin and by depolarization. These results identify specific polypeptides of MW .apprxeq. 250,000 and MW .apprxeq. 32,000 that are components of the Na channel.This publication has 24 references indexed in Scilit:
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