Identification of a gene required for membrane protein retention in the early secretory pathway.
- 1 September 1993
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 90 (17), 8179-8183
- https://doi.org/10.1073/pnas.90.17.8179
Abstract
The yeast SEC12 gene product (Sec12p) is an integral membrane protein required for the protein transport from the endoplasmic reticulum (ER) to the Golgi apparatus. Although this protein is almost exclusively localized in the ER, a significant fraction of Sec12p is modified by an enzyme that resides in the early compartment of the Golgi apparatus, suggesting that Sec12p is cycling between the ER and the early Golgi. We have taken a genetic approach to investigate the retention mechanism of Sec12p. Analysis of mutants that are defective in the retention of the Sec12-Mf alpha 1 fusion protein in the early secretory compartments has identified a gene, RER1. A recessive mutation in RER1 causes mislocalization of the authentic Sec12p as well as two different Sec12 fusion proteins to the late Golgi apparatus and even to the cell surface. However, the rer1 mutant is not defective in the retention of an ER-resident soluble protein, BiP, suggesting that soluble and membrane proteins are retained in the ER by distinct mechanisms.Keywords
This publication has 28 references indexed in Scilit:
- A recycling pathway between the endoplasmic reticulum and the Golgi apparatus for retention of unassembled MHC class I moleculesNature, 1991
- A human homologue of the yeast HDEL receptorNature, 1990
- The ERD2 gene determines the specificity of the luminal ER protein retention systemCell, 1990
- ERD2, a yeast gene required for the receptor-mediated retrieval of luminal ER proteins from the secretory pathwayCell, 1990
- ERS1 a seven transmembrane domain protein fromSaccharomyces cerevisiaeNucleic Acids Research, 1990
- A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus.The Journal of cell biology, 1989
- A membrane glycoprotein, Sec12p, required for protein transport from the endoplasmic reticulum to the Golgi apparatus in yeast.The Journal of cell biology, 1988
- A C-terminal signal prevents secretion of luminal ER proteinsCell, 1987
- Purification and properties of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase from a methanol-utilizing yeast, Candida boidiniiBiochimica et Biophysica Acta (BBA) - Enzymology, 1979
- [76] β-d-Fructofuranoside fructohydrolase from yeastMethods in Enzymology, 1975