Pertussis toxin inhibits chemotactic factor‐induced phospholipase C stimulation and lysosomal enzyme secretion in rabbit neutrophils

Abstract
Pertussis toxin suppressed [32P]polyphosphoinositide breakdown and lysosomal enzyme secretion induced by fMet-Leu-Phe in rabbit neutrophils. Likewise, fMet-Leu-Phe- or leukotriene B4-evoked (3H]inositol trisphosphate accumulation was inhibited by the toxin. These findings, taken together with evidence that pertussis toxin specifically causes inactivation of the guanine nucleotide binding protein (Ni), suggests that guanine nucleotide binding proteins may mediate coupling between calcium-mobilising receptors and phospholipase C-mediated reactions in rabbit neutrophils.

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