Human T lymphocytes recognize a peptide of single point-mutated, oncogenic ras proteins.
Open Access
- 1 January 1991
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 173 (1), 273-276
- https://doi.org/10.1084/jem.173.1.273
Abstract
P21ras proteins are thought to play an important role in cell proliferation and differentiation. Single nucleotide mutations in the encoding cellular proto-oncogenes often result in p21ras proteins with transforming activity. Such activated ras oncogenes have been demonstrated in a variety of human malignancies and also in preneoplastic changes. Using a synthetic peptide corresponding to amino acids 5-16 of mutated p21ras proteins with an exchange of the normal glycine at position 12 by valine, it is shown here that human CD4+ T cells specifically recognize the mutated protein sequence and can be generated as antigen-specific T lymphocyte lines. The fact that these T lines did not crossreact to the sequence of normal p21ras proteins offers new perspectives for specific immunotherapy of human malignancies and even precancerous lesions.Keywords
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