Cytosolic Ca2+-dependent neutral proteinases from rabbit liver: activation of the proenzymes by Ca2+ and substrate.
- 1 January 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (1), 53-56
- https://doi.org/10.1073/pnas.81.1.53
Abstract
Two neutral Ca2+-dependent proteinases, differing in molecular size, were isolated from rabbit liver. Both are recovered as inactive proenzymes that can be converted to the active forms by high (0.1-1.0 mM) concentrations of Ca2+ in the absence of substrate or, in the presence of a protein substrate, by low (1-5 .mu.M) concentrations of Ca2+. The activated proteinases required only 1-5 .mu.M Ca2+ for maximal activity. Substrates hydrolyzed were denatured globin, globin, casein, and to a lesser extent, several extracellular proteins; no digestion was observed with several intracellular cytosolic enzymes tested. Only those proteins that served as substrates were capable of promoting conversion of the pro-enzymes to the active low-Ca2+-requiring proteinases.This publication has 21 references indexed in Scilit:
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