A CASEIN DIGEST MEDIUM FOR TOXIN PRODUCTION BY CLOSTRIDIUM

Abstract

A simplified casein digest–corn steep medium is described that lends itself to large scale use. The advantages stressed are: use of fresh or frozen pancreas as a source of trypsin, a digest that is readily clarified, the clarification of corn steep by steaming in alkaline solution. The product is a medium that supports good growth of many species of bacteria and in which Clostridium botulinum produces large and consistent yields of toxin.