STUDIES ON THE FORMATION OF AN ENZYME-SUBSTRATE COMPLEX BETWEEN MYOSIN AND ADENOSINETRIPHOSPHATE

Abstract

A methodology has been developed by which the concentration of ATP in a reaction system is continuously measured by recording the light emitted by the firefly luciferase-luciferin system. When in such a system, also containing phosphocreatine and creatine kinase, myosin is introduced, the decrease in luminescence is due to the binding of ATP by myosin, since the system could be so designed that all other factors were negligible. The enzyme-substrate binding takes place in a one to one molar ratio.