Equilibrium-binding studies of pig laryngeal cartilage proteoglycans with hyaluronate oligosaccharide fractions
- 1 January 1980
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 185 (1), 107-114
- https://doi.org/10.1042/bj1850107
Abstract
The binding of hyaluronate oligosaccharide fractions to proteoglycans from pig laryngeal cartilage was studied by equilibrium dialysis in dilute solution. Each proteoglycan monomer binds only 1 hyaluronate oligosaccharide molecule [containing .apprx. 18 saccharide residues .**GRAPHIC**. and of number-average molecule weight (.hivin.Mn) 3750]. Kd for interaction between proteoglycan monomer and oligosaccharide .**GRAPHIC**. is 3 .times. 10-8 M at 6.degree. C at I [ionic strength] 0.15-0.5, pH 7.4. Kd has little dependence on temperature, so that Kd at 54.degree. C is 3 .times. 10-7 M under the same conditions. The aggregatability is high at 6.degree. C, falls significantly at 54.degree. C, but much of it can be recovered on cooling to 6.degree. C again, demonstrating reversible denaturation. A method for determining the proportion of the proteoglycan molecules capable of binding to hyaluronate by equilibrium dialysis was compared with gel-chromatographic and ultracentrifugal methods. A hyaluronate oligosaccharide, .**GRAPHIC**. (.hivin.Mn 11,000), could bind more than 1 proteoglycan molecule. Ultracentrifugal data shows that when proteoglycans bind to a hyaluronate of larger size (MW 670,000), an average Kd of 2 .times. 107 M fits the data in 0.5 M guanidine hydrochloride at 20.degree. C.This publication has 13 references indexed in Scilit:
- Affinity binding of the cartilage proteoglycan protein-keratan sulfate core to immobilized hyaluronic acidAnalytical Biochemistry, 1978
- Self-association of proteoglycan subunits from pig laryngeal cartilageBiochemical Journal, 1978
- Polydispersity of cartilage proteoglycans. Structural variations with size and buoyant density of the molecules.Journal of Biological Chemistry, 1977
- Cartilage proteoglycans. Structure and heterogeneity of the protein core and the effects of specific protein modifications on the binding to hyaluronateBiochemical Journal, 1976
- Aggregation of cartilage proteoglycans. II. Oligosaccharide competitors of the proteoglycan-hyaluronic acid interaction.1974
- Aggregation of cartilage proteoglycans. I. The role of hyaluronic acid.1974
- Hyaluronic acid in cartilage and proteoglycan aggregationBiochemical Journal, 1974
- Binding of oligosaccharides of hyaluronic acid to proteoglycans (Short Communication)Biochemical Journal, 1973
- The specific interaction of hyaluronic acid with cartilage proteoglycansBiochimica et Biophysica Acta (BBA) - General Subjects, 1972
- Proteinpolysaccharide complex from bovine nasal cartilage. The function of glycoprotein in the formation of aggregates.1969