A GTP-binding protein of Escherichia coli has homology to yeast RAS proteins.
- 1 December 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (23), 8849-8853
- https://doi.org/10.1073/pnas.83.23.8849
Abstract
The DNA sequence of a gene (era) located immediately downstream of the gene (rnc) encoding ribonuclease III of Escherichia coli was determined and found to encode a protein of 316 amino acid residues. The amino acid sequence of this protein, Era, has significant similarity to the yeast RAS proteins. Overexpression of the Era protein was achieved and GTP cross-linking experiments demonstrated that the protein was indeed capable of binding GTP, as are the yeast and mammalian ras gene products. These data indicate that ras-related sequences occur not only in eukaryotes but also in prokaryotes.Keywords
This publication has 50 references indexed in Scilit:
- Differential activation of yeast adenylate cyclase by wild type and mutant RAS proteinsCell, 1985
- The drosophila ras oncogenes: Structure and nucleotide sequenceCell, 1984
- Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteinsCell, 1984
- Activation of Ki-ras2 gene in human colon and lung carcinomas by two different point mutationsNature, 1983
- Acquisition of transforming properties by alternative point mutations within c-bas/has human proto-oncogeneNature, 1983
- Identification of transforming gene in two human sarcoma cell lines as a new member of the ras gene family located on chromosome 1Nature, 1983
- Mechanism of activation of a human oncogeneNature, 1982
- T24 human bladder carcinoma oncogene is an activated form of the normal human homologue of BALB- and Harvey-MSV transforming genesNature, 1982
- Human EJ bladder carcinoma oncogene is homologue of Harvey sarcoma virus ras geneNature, 1982
- Empirical Predictions of Protein ConformationAnnual Review of Biochemistry, 1978