Inhibition of Macrophage Migration by Virus‐Induced Interferon Preparations1

Abstract

A preparation of mouse [fibroblast] L cell interferon induced by Newcastle disease virus (NDV) possessed interferon activity and inhibitory activity on migration of guinea pig peritoneal macrophages (MIF activity). These activities were also observed in a preparation of human leukocyte interferon induced by NDV. The interferon and MIF activities shared common characteristics in the dose response, time course of in vitro production, thermal stability, sensitivity to trypsin and periodate, and elution pattern in CM-Sephadex column chromatography. Gel filtration pattern with Sephadex G-100 showed 2 separate peaks. Fractions collected from the 1st peak, corresponding to a MW of .apprx. 45,000, had only the MIF activity; those collected from the 2nd peak, corresponding to a MW of .apprx. 30,000, had the interferon and MIF activities. A preparation of mouse brain interferon induced by Japanese encephalitis virus had a much weaker MIF activity than the L cell interferon, although these preparations were equal in interferon activity (5000 units/ml).