Functionality of nitrated acetylcholine receptor: The two-step formation of nitrotyrosines reveals their differential role in effectors binding
- 9 April 2005
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 579 (12), 2643-2647
- https://doi.org/10.1016/j.febslet.2005.03.084
Abstract
International audienceThe presence of nitrotyrosines is associated with several neurodegenerative pathologies. We evaluated the functionality of the nicotinic acetylcholine receptor possessing nitrotyrosines. The spectrum of the nitrated receptor displays an absorption band characteristic of ortho-nitrophenol. The presence of carbamylcholine in the agonist site prevented the effect of nitration by tetranitromethane in some conditions. The nitration occurred with two discrete steps and pointed out the differential involvement of tyrosines in the binding of acetylcholine and neurotoxin. We concluded that at least two residues involved in agonist binding can be nitrated, which bring similar contributions to the binding energy of the neurotransmitter. Cop. 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reservedKeywords
This publication has 24 references indexed in Scilit:
- NO-dependent protein nitration: a cell signaling event or an oxidative inflammatory response?Trends in Biochemical Sciences, 2003
- The Binding Site of Acetylcholine ReceptorAnnals of the New York Academy of Sciences, 2003
- The nicotinic receptor ligand binding domainJournal of Neurobiology, 2002
- α 7 -Nicotinic Acetylcholine Receptors on Cerebral Perivascular Sympathetic Nerves Mediate Choline-Induced Nitrergic Neurogenic VasodilationCirculation Research, 2002
- Cation–π interactions in ligand recognition and catalysisTrends in Pharmacological Sciences, 2002
- Just in time and place: NOS/NO system assembly in neuromuscular junction formationMicroscopy Research and Technique, 2001
- Superoxide Reacts with Nitric Oxide to Nitrate Tyrosine at Physiological pH via PeroxynitriteJournal of Biological Chemistry, 2000
- Nicotinic Receptors at the Amino Acid LevelAnnual Review of Pharmacology and Toxicology, 2000
- Interaction of modified neurotoxins fromNaja nigricolliswith the nicotinic acetylcholine receptor fromTorpedo marmorataA Raman spectroscopy studyFEBS Letters, 1991
- Increased agonist affinity is induced in tetranitromethane-modified muscarinic receptorsBiochemistry, 1985