In vitro resorptive activity of isolated chick osteoclasts: Effects of carbonic anhydrase inhibition
Open Access
- 1 January 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Bone and Mineral Research
- Vol. 6 (1), 61-66
- https://doi.org/10.1002/jbmr.5650060111
Abstract
A potent inhibitor of carbonic anhydrase, 5-[3-hydroxybenzoyl]thiophene-2-sulfonamide (HTS), was shown to cause a 37% reduction in the area of resorption pits formed by isolated chick osteoclasts when used at a dose of 10−7 M. HTS at doses of 10−9 and 10−7 M was also effective in reducing acid formation by the osteoclasts (14 and 36%, respectively). Additionally, the effect of HTS was found to be readily reversed by removing the agent, showing that it does not exert a toxic effect on the cells. This study indicates that the inhibitory effect of HTS on bone resorption is at the level of the acid-forming mechanism in osteoclasts and supports the view that carbonic anhydrase has a central role in the process.Keywords
Funding Information
- NIH (DE 04345)
- Biomedical Research ((S07 RR07161))
- University of Maine from the National Institutes of Health
- NASA (NAGW-1196)
- Center for Cell Research at Penn State
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