Immune Hemolysis and the Functional Properties of the Second (C2) and Fourth (C4) Components of Complement

Abstract

Treatment of guinea pig or human C2 with trypsin in the presence of EAC4 leads to the formation of EAC. The kinetics of EAC generation, the nature of the C2 dose response curve and the nature of the decay curve of SA all indicated that EAC formed by the action of trypsin on C2 are not distinguishable from EAC generated by the interaction of EAC1̄4 and C2. The data support the hypothesis that C1̄ is a trypsin-like enzyme.