Location of the Aspartase Gene (aspA) on the Linkage Map of Escherichia coli K12

Abstract
The aspartase (L-aspartate ammonia-lyase, EC 4.3.1.1) structural gene, aspA, was mapped by 2-factor and 3-factor transductional crosses using phage P1. Cotransduction frequencies between aspA and other markers were: ampA (69%); frdA (48-67%); mel (35%); purA (17%); fdp (1.6%). The sequence of markers within the corresponding segment (91-95 min) of the E. coli linkage map was mel-aspA-ampA-frdA-purA-fdp.