Characterization of proteins phosphorylated by the cAMP‐dependent protein kinase of bovine heart mitochondria

Abstract
Characterization of two mitochondrial proteins of M 1 42 and 18 kDa, respectively, phosphorylated by the cAMP–dependent protein kinase of bovine heart mitochondria (mtPKA), is presented. A 42 kDa protein is found to be loosely associated to complexes I, III and IV of the respiratory chain and complex V (ATP synthase) in the inner mitochondrial membrane. An 18 kDa protein is associated to complex I in the inner membrane and in a purified preparation of this complex where it can be phosphorylated by the isolated catalytic subunit of PKA.