Proteus mirabilis Phage 5006M: a Physical Characterization
- 1 November 1979
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 45 (2), 389-395
- https://doi.org/10.1099/0022-1317-45-2-389
Abstract
This report deals with physical characterization of the generalized transducing Proteus mirabilis phage 5006M. The morphology of the phage is presented, the buoyant density was determined (1.491 g/ml) and the G + C content of the phage DNA was found to be 44%. The phage genome has a length of 14.8 µm and mol. wt. of 30.7 × 106. Denaturation mapping revealed non-random circular permutation of the phage DNA. The genome exhibits 3.6% terminal redundancy as shown by homoduplex analysis. The existence of concatemeric precursors of phage 5006M DNA is inferred and the results are interpreted in terms of a sequential headful packaging mechanism.This publication has 4 references indexed in Scilit:
- EcoRI restriction endonuclease cleavage site map of bacteriophage P22 DNAJournal of Molecular Biology, 1978
- DNA maturation by the “headful” mode in bacteriophage T1Journal of Molecular Biology, 1977
- The Morphology of Proteus BacteriophagesJournal of General Microbiology, 1965
- Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperatureJournal of Molecular Biology, 1962