Isolation and characterization of the opioid peptides from rat pituitary: beta-endorphin.
- 1 November 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (11), 4969-4972
- https://doi.org/10.1073/pnas.74.11.4969
Abstract
.beta.-Endorphin was isolated from 200 rat pituitaries by means of high-performance column chromatography, using sensitive fluorometric methods and a radioreceptor assay for opioid activity. The .beta.-endorphin was characterized as to molecular weight, amino acid composition and mapping of tryptic peptides by a new microtechnique. Rat .beta.-endorphin is identical to camel and sheep .beta.-endorphin. .alpha.-Endorphin, Met-enkephalin, a nonpeptide morphine-like compound (MLC), and an additional unidentified opioid compound were detected in the extract of rat pituitary.This publication has 7 references indexed in Scilit:
- Binding of the endogenous nonpeptide morphine-like compound to opiate receptors.Proceedings of the National Academy of Sciences, 1977
- Homology between a prolyl hydroxylase subunit and a tissue protein that crossreacts immunologically with the enzyme.Proceedings of the National Academy of Sciences of the United States of America, 1977
- Isolation and characterization of the opioid peptides from rat pituitary: beta-lipotropin.Proceedings of the National Academy of Sciences, 1977
- Antibodies as a means of isolating and characterizing biologically active substances: presence of a non-peptide, morphine-like compound in the central nervous system.Proceedings of the National Academy of Sciences, 1976
- Isolation and structure of an untriakontapeptide with opiate activity from camel pituitary glands.Proceedings of the National Academy of Sciences of the United States of America, 1976
- Automatic monitoring of primary amines in preparative column effluents with fluorescamineAnalytical Biochemistry, 1975
- Amino acid analysis with fluorescamine at the picomole levelArchives of Biochemistry and Biophysics, 1973