Quantitation of Ineffective Erythropoiesis from the Incorporation of [15N]Delta-aminolaevulinic Acid and [15N]Glycine into Early Labelled Bilirubin: I. NORMAL SUBJECTS

Abstract

SUMMARY The incorporation of [¹⁵N]glycine into early labelled bilirubin and haemoglobin haem was measured in four haematologically normal subjects, using the clearance of [¹⁴C] bilirubin to measure total bilirubin production rate. Hepatic haem turnover was calculated from the incorporation of [¹⁵N]δ-aminolaevulinic acid into early labelled bilirubin. From the experimental data and previously published data in normal subjects a method is derived for the quantitation of ineffective erythropoiesis which can be applied to similar studies in patients with haemato-logical disorders.