Electrical Stimulation of the Substantia Nigra and Changes of 2‐Phenylethylamine Synthesis in the Rat Striatum
- 1 January 1991
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 56 (1), 213-220
- https://doi.org/10.1111/j.1471-4159.1991.tb02583.x
Abstract
In rats pretreated with deprenyl (2 mg/kg), electrical stimulation of the left substantia nigra produced an increase in the concentrations of 3,4-dihydroxyphenylacetic acid and homovanillic acid in the left striatum by 57 and 45%, but the levels of 2-phenylethylamine and p-tyramine decreased by 22 and 41%, respectively, as compared with those in the right striatum. The administration of α-methyl-p-tyrosine (1.25 mg/kg, i.p.), a tyrosine hydroxylase inhibitor, 1 h before nigral stimulation, did not affect the concentration of 2-phenylethylamine in unstimulated striata but prevented the stimulation-induced decrease in the concentration of 2-phenylethylamine. Neither stimulation nor α-methyl-p-tyrosine affected the activity of monoamine oxidase A or B, and stimulation did not produce any change in striatal blood flow, a finding demonstrating that the changes in the rate of accumulation of 2-phenylethylamine were not due to changes in catabolism or removal of 2-phenylethylamine from the brain. These experiments demonstrate that the rate of synthesis of striatal 2-phenylethylamine is decreased following nigral stimulation and that this effect is blocked after partial inhibition of tyrosine hydroxylase. This suggests that 2-phenylethylamine is present in tyrosine hydroxylase-containing neurons and therefore supports the coexistence of 2-phenylethylamine and dopamine in the nigrostriatal pathway.Keywords
This publication has 44 references indexed in Scilit:
- The in vivo distribution of 99Tcm-HM-PAO in normal manNuclear Medicine Communications, 1986
- Kinetic Measurements of the Turnover Rates of Phenylethylamine and Tryptamine In Vivo in the Rat BrainJournal of Neurochemistry, 1980
- THE EFFECTS OF RESERPINE AND 6‐HYDROXYDOPAMINE ON THE CONCENTRATIONS OF SOME ARYLALKYLAMINES IN RAT BRAINBritish Journal of Pharmacology, 1977
- Identification and Distribution of β-Phenylethylamine in the RatCanadian Journal of Biochemistry, 1973
- Preparation and properties of a homogeneous aromatic l-amino acid decarboxylase from hog kidneyArchives of Biochemistry and Biophysics, 1970
- Postnatal changes in free amino acid pool of rabbit brainBrain Research, 1967
- Demonstration and mapping out of nigro-neostriatal dopamine neuronsLife Sciences, 1964
- Effects of decarboxylase inhibition on the biosynthesis of brain monoaminesLife Sciences, 1962
- Effect of Reserpine on the Storage of Catechol Amines in Brain and Other TissuesActa Physiologica Scandinavica, 1961
- Substrate specificity of amino-acid decarboxylasesBiochimica et Biophysica Acta, 1950