Neutrophil function screenlng in patients with chronic granulomatous disease by a flow cytometric method
- 1 January 1992
- Vol. 13 (6), 615-620
- https://doi.org/10.1002/cyto.990130609
Abstract
Neutrophils from patients with chronic granulomatous disease (CGD) fail to produce a significant oxidative burst following stimulation. We have evaluated the use of flow cytometry and the dye 2′,7′dichlorofluorescein diacetate (DCF) for routine screening for deficiencies of neutrophil oxidative burst. A range for DCF fluorescence for phorbol myristate acetate stimulated and non‐stimulated neutrophils was established based on data from 52 healthy adults. Samples from three patients with suspected neutrophil dysfunction, three patients with X‐linked CGD, and one patient with autosomal recessive (AR) CGD were evaluated with both the DCF assay and the quantitative nitroblue tetrazolium dye reduction (NBT) test. For the DCF test, the ratio of mean fluorescence intensity of stimulated to non‐stimulated neutrophils was 50 for healthy individuals. With the DCF test, two populations of neutrophils could be identified in samples from four carriers of X‐linked CGD, although two carriers of AR CGD had NBT and DCF results in the normal range. Our data suggest the DCF test is a sensitive and convenient method for detecting CGD.Keywords
This publication has 20 references indexed in Scilit:
- Genetic Variants of Chronic Granulomatous Disease: Prevalence of Deficiencies of Two Cytosolic Components of the NADPH Oxidase SystemNew England Journal of Medicine, 1989
- Chronic granulomatous disease due to a defect in the cytosolic factor required for nicotinamide adenine dinucleotide phosphate oxidase activation.JCI Insight, 1988
- Cloning the gene for an inherited human disorder—chronic granulomatous disease—on the basis of its chromosomal locationNature, 1986
- Activation of human neutrophil nicotinamide adenine dinucleotide phosphate, reduced (triphosphopyridine nucleotide, reduced) oxidase by arachidonic acid in a cell-free system.JCI Insight, 1985
- Absence of Cytochrome b-245in Chronic Granulomatous DiseaseNew England Journal of Medicine, 1983
- Pyridine nucleotide-dependent superoxide production by a cell-free system from human granulocytes.JCI Insight, 1975
- Complete Deficiency of Leukocyte Glucose-6-Phosphate Dehydrogenase with Defective Bactericidal ActivityJCI Insight, 1972
- Studies of the Metabolic Activity of Leukocytes from Patients with a Genetic Abnormality of Phagocytic Function*JCI Insight, 1967
- The role of the phagocyte in host-parasite interactions: VII. Di- and triphosphorydine nucleotide kinetics during phagocytosisBiochimica et Biophysica Acta (BBA) - General Subjects, 1967
- Biochemical Aspects of PhagocytosisNature, 1961