Some Modern Concepts in Reversed-Phase Liquid Chromatography on Chemically Bonded Alkyl Stationary Phases

Abstract

Some important aspects of modern reversed-phase liquid chromatography are reviewed. The general characteristics of this separation mode, noting particularly its versatility, are discussed in terms of its advantages and limitations. The performance that can now be achieved with modern columns is demonstrated. The importance of stationary phase effects is illustrated with some practical examples. The role of the mobile phase is discussed with emphasis on ways of improving peak shape for problematic samples. Finally, some new application areas, such as the use of special mobile phase chemical additives, are briefly described.