Cyclic AMP‐dependent protein kinase and Ca2+‐calmodulin stimulate the formation of polyphosphoinositjdes in a sarcoplasmic reticulum preparation of rabbit heart
- 15 October 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 176 (1), 235-238
- https://doi.org/10.1016/0014-5793(84)80948-5
Abstract
A rabbit heart membrane fraction enriched in sarcoplasmic reticulum was incubated in a reaction mixture containing [γ-32P]ATP. The catalytic subunit of cyclic AMP-dependent protein kinase enhanced the 32P-labelling of both phosphatidylinositol-4-phosphate and phosphatidylinositol-4,5-bisphosphate. Ca2+-calmodulin also increased the 32P-incorporation into both polyphosphoinositides. Upon SDS gel-electrophoretic analysis of the membrane proteins, phospholamban was found to be concurrently phosphorylated by the exogenous catalytic subunit as well as by an endogenous Ca2+-calmodulm-dependent protein kinase.Keywords
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