Separation of the Subunits of Denatured Collagens and Gelatins with Starch Gel Electrophoresis.

Abstract

Gelatinized soluble collagens could be divided into at least six distinct subunits by starch gel electrophoresis. The remnants of these subunits of collagen could be detected also in certain commercial gelatins. At reconstitution some subunits disappeared and a new fraction was demonstrated. The salt-soluble and acid-soluble collagens from rat skin did not differ markedly in their subunit composition, but neutral salt-soluble collagens from lathyritic rats yielded [alpha]-units only.