The major bile acids in samples of bile as determined with the aid of gas-liquid chromatography were cholic acid, chenodcoxycholic and deoxycholic acid. Regarding the proportions of the different bile acids, the results from this small group agree with those of Wootton and Wiggins(5). Isaksson(6) and Sjövall(7.8). Deoxycholic acid seemed to disappear in obstructive jaundice. In hepatic bile obtained from patients without jaundice only very small amounts of deoxycholic acid were found. Similar results have been reported by Sjövall (7) and Rudman and Kendall(9).