Renal Filtration and Catabolism of Complement Protein D
- 14 February 1985
- journal article
- research article
- Published by Massachusetts Medical Society in New England Journal of Medicine
- Vol. 312 (7), 395-399
- https://doi.org/10.1056/nejm198502143120702
Abstract
Complement protein D, a serine protease participating in the formation of the C3 convertase of the alternative complement pathway, has the lowest molecular weight (23,750) and serum concentration of all complement proteins. In normal serum, D is the rate-limiting protease of the alternative pathway of complement activation. We report that the serum concentrations of D in 20 patients with chronic renal failure (mean ±S.D., 0.42±0.28 mg per deciliter) and in 16 patients on long-term dialysis (1.53±0.39 mg per deciliter) were significantly higher (P<0.001) than in 22 healthy adults (0.18±0.04 mg per deciliter). In chronic renal failure the serum concentration of D correlated with that of creatinine (r = 0.75, P<0.001). The serum concentrations of D found in patients with renal failure reached and in some cases exceeded those at which the protease is no longer rate-limiting. Thus, enhanced activity of the alternative pathway of complement should be expected in patients with advanced renal failure.This publication has 20 references indexed in Scilit:
- Amino acid sequence of human D of the alternative complement pathwayBiochemistry, 1984
- Quantitation of complement factor D in human serum by a solid-phase radioimmunoassayJournal of Immunological Methods, 1984
- Amino acid sequence of human factor D of the complement systemFEBS Letters, 1984
- Ultrasensitive Stain for Proteins in Polyacrylamide Gels Shows Regional Variation in Cerebrospinal Fluid ProteinsScience, 1981
- Amino-terminal sequence of human factor B of the alternative complement pathway and its cleavage fragments, Ba and BbBiochemistry, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Mechanism of action of factor D of the alternative complement pathway.The Journal of Experimental Medicine, 1978
- HUMAN COMPLEMENT METABOLISMMedicine, 1975
- PROPERDIN FACTOR D: CHARACTERIZATION OF ITS ACTIVE SITE AND ISOLATION OF THE PRECURSOR FORMThe Journal of Experimental Medicine, 1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970