A Sensitive Isotope Selective Nondispersive Infrared Spectrometer for 13CO2 and 12CO2 Concentration Measurements in Breath Samples
- 1 July 1994
- journal article
- research article
- Published by Taylor & Francis in Isotopenpraxis Isotopes in Environmental and Health Studies
- Vol. 30 (2-3), 247-251
- https://doi.org/10.1080/00211919408046740
Abstract
We present a nondispersive infrared spectrometer (NDIRS) for the measurement of the 13CO2/12CO2-ratio in breath samples. A commercial NDIR spectrometer for CO2 concentration measurements in industrial process control was modified using two separate optical channels for the 13CO2 and 12CO2 detection. Cross interference due to overlapping absorption lines of both isotopic gases was successfully eliminated. The sensitivity of this device is ± 0.4‰ of the 13CO2/12CO2-ratio in a range of 2.5 to 5% of total CO2. This is sufficient for biomedical applications. Our spectrometer is small in size, cheap and simple to operate and thus a true alternative to isotope ratio mass spectrometers (IRMS). Several biomedical applications with breath samples were demonstrated and were compared in very good agreement with IRMS.Keywords
This publication has 4 references indexed in Scilit:
- Biomedical Application of an Isotope Selective Nondispersive Infrared Spectrometer for 13CO2 and 12CO2 Concentration Measurements in Breath SamplesIsotopenpraxis Isotopes in Environmental and Health Studies, 1994
- Measurement of carbon-13 dioxide/carbon-12 dioxide abundance by nondispersive infrared heterodyne ratiometry as an alternative to gas isotope ratio mass spectrometryAnalytical Chemistry, 1986
- Determination of Isotope Ratios, e.g., in Tracer Work, by an Infrared Absorption MethodThe Journal of Chemical Physics, 1951
- III. Action of an intermittent beam of radiant heat upon gaseous matterProceedings of the Royal Society of London, 1881