Pre-isoflavonoid stages in the biosynthesis of amorphigenin: ring-D formation and ring-A migration

Abstract

Despite low incorporations of doubly labelled [¹³C]acetate during the biosynthesis of amorphigenin by Amorpha fruticosa seedlings, it has been possible to solve the ring-D folding by an INADEQUATE n.m.r. experiment; new proposals are made for the mechanism of the enzyme catalysed flavanone–isoflavone transformation step.