Peroxidase Staining Combined with Autoradiography for Study of Eosinophilic Granules
- 1 January 1962
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 37 (4), 243-247
- https://doi.org/10.3109/10520296209117745
Abstract
Giemsa staining and a peroxidase reaction were applied to blood films in conjunction with autoradiography to establish the types of granulocytes that stain differentially with the benzidine-peroxidase reaction. Differential counts made on Ciemsa-stained and peroxidase-stained autoradiograms were compared. In T. spiralis-infected rats with an elevated eosinophil count, as judged by Giemsa staining, the percentage of granulocytes that stained more intensely with peroxidase was increased. The results suggested that the eosinophils were the intensely peroxidase-positive cells. Blood smears were stained for peroxidase before being coated with NTB2 liquid emulsion. Although the blue color of the peroxidase reaction faded during photographic development, the color redeveloped when peroxidase-stained autoradiograms were stained once again after photographic development. It was found necessary to stain for peroxidase both before and after autoradiography. The correlation of Giemsa-stained and peroxidase-stained autoradiograms indicated that the peroxidase stain can be combined with autoradiography to obtain authentic results.Keywords
This publication has 3 references indexed in Scilit:
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- Uptake of Radioactive Sulfate by Elements of the Blood and the Bone Marrow of RatsAmerican Journal of Physiology-Legacy Content, 1955
- Giemsa Staining of Autoradiograms Prepared with Stripping FilmStain Technology, 1955