THE EFFECT OF ESTERIFICATION OF PROTEIN CARBOXYL GROUPS ON THE STAINING OF BACTERIAL CELLS
- 31 August 1949
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 58 (3), 347-350
- https://doi.org/10.1128/jb.58.3.347-350.1949
Abstract
Heat-fixed smears of 8 organisms were immersed for 1 week in methyl alcohol containing 0.05 [image] HC1. The resultant esterification of the cell protein greatly reduced the affinity of the cells for basic dyes, while affinity for acid dyes was either unchanged or increased as compared to control slides. These results give additional proof that basic dyes stain bacterial protoplasm by combination with free carboxyl groups.Keywords
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