Increased detectability of somatic changes in the DNA from human tumours after probing with ?synthetic? and ?genome-derived? hypervariable multilocus probes
- 1 December 1989
- journal article
- research article
- Published by Springer Nature in Human Genetics
- Vol. 84 (1), 35-40
- https://doi.org/10.1007/bf00210667
Abstract
DNA fingerprinting with two minisatellite (33.15, M13) and two simple repeat probes [(GACA)4, (CAC)4/ (GTG)5] was performed to screen for somatic changes in the DNA from various solid human tumours in comparison with constitutional DNA from the same patient. Loss of bands or changes in band intensitities were observed. Together the probes 33.15 and (CAC)5/(GTG)5 detected deviating fingerprint patterns in 63% of the colorectal carcinomas investigated. In mammary and stomach carcinomas, only 1/11 and 2/11 tumours, respectively, showed differences with either of the three probes, 33.15, (GACA)4 and (CAC)5/(GTG)5.This publication has 39 references indexed in Scilit:
- Assessment of clonality in gastrointestinal cancer by DNA fingerprinting.JCI Insight, 1988
- DNA fingerprinting and 'scientific' whalingNature, 1988
- Optimized oligonucleotide probes for DNA fingerprintingElectrophoresis, 1988
- Hypervariable minisatellite regions are sites for crossing-over at meiosis in manCytogenetic and Genome Research, 1988
- DNA fingerprints applied to animal and bird populationsNature, 1987
- A Sequence in M13 Phage Detects Hypervariable Minisatellites in Human and Animal DNAScience, 1987
- An evaluation of DNA fingerprinting for forensic purposesElectrophoresis, 1987
- Mutagens, oncogenes and cancerTrends in Genetics, 1986
- Gene amplification in cultured animal cellsCell, 1984
- GENE AMPLIFICATIONAnnual Review of Biochemistry, 1984