Production of L-alanyl-L-glutamine by immobilizedEscherichia coliexpressing amino acid ester acyltransferase
- 1 August 2020
- journal article
- research article
- Published by Springer Nature in Applied Microbiology and Biotechnology
- Vol. 104 (16), 6967-6976
- https://doi.org/10.1007/s00253-020-10752-3
Abstract
Production of Ala-Gln by theE. coliexpressing alpha-amino acid ester acyltransferase was a promising technical route due to its high enzyme activity, but the continuous production ability still needs to improve. Therefore, the immobilizedE. coliexpressing alpha-amino acid ester acyltransferase was applied for the continuous production of Ala-Gln. Four materials were selected as embedding medium for the whole cell entrapment of recombinant bacteria. Calcium alginate beads were found to be the most proper entrapment carrier for production of Ala-Gln. The temperature, pH, and repeatability of the immobilized cell were compared with free cells. Results showed that immobilization cell could maintain a wider range of temperature/pH and better stability than free cell (20-35 degrees C/pH 8.0-9.0, and 25 degrees C/pH 8.5, respectively). On this basis, continuous production strategy was put forward by filling the immobilized cell in the tubular reactor with multiple control conditions. The Ala-Gln by immobilization cell achieved the productivity of 2.79 mg/(min*mL-CV) without intermittent time. Consequently, these findings suggest that the immobilization technique has potential applications in the production of Ala-Gln by biotechnological method.Keywords
This publication has 35 references indexed in Scilit:
- Synthesis of biodiesel in supercritical alcohols and supercritical carbon dioxideFuel, 2010
- Microbial cells as catalysts for stereoselective red–ox reactionsBiotechnology Advances, 2009
- c-Myc suppression of miR-23a/b enhances mitochondrial glutaminase expression and glutamine metabolismNature, 2009
- Synthesis and Mechanism Studies on Amide Bond Formation by Hexamethylphosphoramide (HMPA)Phosphorus, Sulfur, and Silicon and the Related Elements, 2008
- Fermentative Production ofl-Alanyl-l-Glutamine by a Metabolically EngineeredEscherichia coliStrain Expressingl-Amino Acid α-LigaseApplied and Environmental Microbiology, 2007
- Production of Chitosan Oligosaccharides at High Concentration by Immobilized ChitosanaseFood Science and Technology Research, 2006
- A novel and efficient enzymatic method for the production of peptides from unprotected starting materialsJournal of Biotechnology, 2005
- Environmental applications of immobilized microbial cells: A reviewJournal of Industrial Microbiology & Biotechnology, 1996
- The immobilization of whole cells: Engineering principlesChemical Engineering Science, 1985
- Protection of Amide-Nitrogen for Peptide Synthesis. A Novel Synthesis of Peptides Containing C-Terminal GlutamineBulletin of the Chemical Society of Japan, 1961