Purification and Immunological Characterization of the Major Internal Protein of the RD-114 Virus

Abstract

The major internal protein of the RD-114 virus that appeared in a human tumor cell line, RD, after passage through fetal cats was purified by isoelectric focusing and used to prepare antibody in guinea pigs. The protein has a molecular weight of 33,500 in sodium dodecyl sulfate-polyacrylamide gels, and an isoelectric point of 9.1. This contrasts with a molecular weight of 25,000 and an isoelectric point of 8.3 obtained previously for the major protein of cat C-type viruses. The RD-114 protein carries determinants common to all mammalian C-type viruses but not species-specific determinants of cat, mouse, rat, hamster, chicken, and Russell's viper C-type viruses. In addition, two other proteins found in cat virus preparations were not detected in the RD-114 virus. Antiserum to the RD-114 viral protein proved highly specific by complement fixation and gel diffusion assays, and, in addition to the above mentioned mammalian viruses, the antiserum did not react with concentrates of the Woolly monkey or Gibbon ape C-type viruses. These data support the conclusion that the RD-114 virus is a human virus activated by passage through fetal cats. Until additional isolates are made from human cells, the identity of the RD-114 virus as a human C-type tumor virus cannot be fully established; however, this would be highly probable if the species-specific group-specific antigen of RD-114 could be demonstrated in human tumor and/or embryonic tissues.