ANALYSIS OF BIOLOGICAL AND IMMUNOLOGICAL ACTIVITIES IN THE TWO POOLS OF LH RELEASED DURING CONSTANT INFUSION OF LUTEINIZING HORMONE-RELEASING HORMONE (LHRH) IN MEN

Abstract
In response to constant infusion of a submaximal dose of LHRH (0.2 ug/min for 4 hours), luteinizing hormone (LH) was released in a biphasic pattern in 6 normal men. When the in vitro biologic activity of plasma LH was compared to the immunologic activity throughout the response to LHRH, the B/I ratio remained unchanged (3.6 ± 0.5, mean ± SE). Thus, in men the biologic activity of stored LH (acutely releasable) is not different from the presumably newly synthesized hormone that is released as the second pool during prolonged LHRH infusion.