A continuous recording technique for the measurement of carbon dioxide, and its application to mitochondrial oxidation and decarboxylation reactions
- 1 June 1967
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 103 (3), 677-691
- https://doi.org/10.1042/bj1030677
Abstract
A technique is described for continuously recording the concentration of carbon dioxide in mitochondrial suspensions. The oxidation of palmitoyl-carnitine by rat-liver mitochondria inhibits the oxidation of pyruvate and isocitrate, and stimulates the carboxylation of pyruvate. These effects of palmitoylcarnitine oxidation are reversed by the uncoupling agent pentachlorophenol. The effects of palmitoylcarnitine oxidation and pyruvate oxidation on the acylation of mitochondrial coenzyme A and reduction of nicotinamide nucleotides were measured. Control mechanisms are discussed for the interactions between palmitoylcarnitine oxidation and the tricarboxylic acid cycle in rat-liver mitochondria.This publication has 32 references indexed in Scilit:
- Paths of carbon in gluconeogenesis and lipogenesis: the role of mitochondria in supplying precursors of phosphoenolpyruvate.Proceedings of the National Academy of Sciences, 1965
- Nicotinamide Adenine Dinucleotide-specific Isocitric DehydrogenaseJournal of Biological Chemistry, 1964
- ZUM MECHANISMUS DER GLUCONEOGENESE UND IHRER STEUERUNG .I. QUANTITATIVE BESTIMMUNG DER PYRUVATCARBOXYLASE IN ROHEXTRAKTEN DER RATTENLEBER1964
- The Effect of Adenylic Acid on Yeast Nicotinamide Adenine Dinucleotide Isocitrate Dehydrogenase, a Possible Metabolic Control MechanismJournal of Biological Chemistry, 1963
- CARNITINE IN INTERMEDIARY METABOLISM - METABOLISM OF FATTY ACID ESTERS OF CARNITINE BY MITOCHONDRIA1962
- The oxidation of glutamate by rat-heart sarcosomesBiochimica et Biophysica Acta, 1960
- Rapid measurement of the tension of carbon dioxide in blood.1957
- The Respiratory Chain and Oxidative PhosphorylationPublished by Wiley ,1956
- Biochemistry of fluoroacetate poisoning: the effect of fluorocitrate on purified aconitaseBiochemical Journal, 1954
- ENZYMATIC SYNTHESIS OF CITRIC ACID .2. CRYSTALLINE CONDENSING ENZYME1951